研究業績集

2024年

• Generation and validation of antibody 42B1 recognizing galactose-deficient IgG for diagnosis of chronic inflammatory diseases Morishima N,Iwaisako M,Kamada Y,Nakano M, Shiida M, Ono T, Sonoda R, Uemura R, Sakon D, Shimosaka M, Takamatsu S, Kondo J, Yoshihara T, Shinzaki S, Mita M, Takehara T, Kumada T, Yamada M, Miyoshi E Clinica Chimica Acta 2024 November, accepted
• Serum levels of the N-terminal fragment of connective tissue growth factor is a novel biomarker for chronic pancreatitis Morishima N, Kamada Y, Ota H, Iwagami Y, Takahashi H, Shimosaka M, Sakon D, Kondo J, Yamada M, Kumada T, Eguchi H, Miyoshi E. Pract Lab Med. 2024 May 18;40:e00402. doi: 10.1016/j.plabm.2024.e00402.
• The S1 spike protein of SARS-CoV-2 upregulates the ERK/MAPK signaling pathway in DC-SIGN-expressing THP-1 cells Johnson EL, Ohkawa Y, Kanto N, Fujinawa R, Kuribara T, Miyoshi E, Taniguchi N. Cell Stress Chaperones. 2024 Apr;29(2):227-234. doi: 10.1016/j.cstres.2024.03.002.
• Dynamic movement of the Golgi unit and its glycosylation enzyme zones Harada A, Kunii M, Kurokawa K, Sumi T, Kanda S, Zhang Y, Nadanaka S, Hirosawa KM, Tokunaga K, Tojima T, Taniguchi M, Moriwaki K, Yoshimura SI, Yamamoto-Hino M, Goto S, Katagiri T, Kume S, Hayashi-Nishino M, Nakano M, Miyoshi E, Suzuki KGN, Kitagawa H, Nakano A. Nat Commun. 2024 May 27;15(1):4514. doi: 10.1038/s41467-024-48901-1.
• Core-fucose-specific Pholiota squarrosa lectin decreased hepatic inflammatory macrophage infiltration in steatohepatitis mice Kamada Y, Ueda Y, Matsuno E, Matsumoto R, Akita M, Takamatsu S, Miyoshi E. Glycoconj J. 2024 Oct;41(4-5):267-278. doi: 10.1007/s10719-024-10163-w. Epub 2024 Sep 9.
• Development of a FUT8 Inhibitor with Cellular Inhibitory Properties Manabe Y, Takebe T, Kasahara S, Hizume K, Kabayama K, Kamada Y, Asakura A, Shinzaki S, Takamatsu S, Miyoshi E, García-García A, Vakhrushev SY, Hurtado-Guerrero R, Fukase K. Angew Chem Int Ed Engl. 2024 Sep 28:e202414682. doi: 10.1002/anie.202414682.
• De Novo Glycan Display on Cell Surfaces Using HaloTag: Visualizing the Effect of the Galectin Lattice on the Lateral Diffusion and Extracellular Vesicle Loading of Glycosylated Membrane Proteins Miura A, Manabe Y, Suzuki KGN, Shomura H, Okamura S, Shirakawa A, Yano K, Miyake S, Mayusumi K, Lin CC, Morimoto K, Ishitobi J, Nakase I, Arai K, Kobayashi S, Ishikawa U, Kanoh H, Miyoshi E, Yamaji T, Kabayama K, Fukase K. J Am Chem Soc. 2024 Aug 14;146(32):22193-22207. doi: 10.1021/jacs.4c02040. Epub 2024 Jul 4.
• Accuracy of the Enhanced Liver Fibrosis Test in Patients With Type 2 Diabetes Mellitus and Its Clinical Implications Arai T, Takahashi H, Seko Y, Toyoda H, Hayashi H, Yamaguchi K, Iwaki M, Yoneda M, Shima T, Fujii H, Morishita A, Kawata K, Tomita K, Kawanaka M, Yoshida Y, Ikegami T, Notsumata K, Oeda S, Atsukawa M, Kamada Y, Sumida Y, Fukushima H, Miyoshi E, Aishima S, Okanoue T, Itoh Y, Nakajima A; Japan Study Group of Nonalcoholic Fatty Liver Disease (JSG-NAFLD). Clin Gastroenterol Hepatol.2024 Apr;22(4):789-797.e8. doi: 10.1016/j.cgh.2023.11.022. Epub 2023 Dec 2.
• Clonal Origin and Lineage Ambiguity in Mixed Neuroendocrine Carcinoma of the Uterine Cervix Masuda M, Iida K, Iwabuchi S, Tanaka M, Kubota S, Uematsu H, Onuma K, Kukita Y, Kato K, Kamiura S, Nakajima A, Coppo R, Kanda M, Yoshino K, Ueda Y, Morii E, Kimura T, Kondo J, Okada-Hatakeyama M, Hashimoto S, Inoue M. Am J Pathol. 2024 Mar;194(3):415-429. doi: 10.1016/j.ajpath.2023.11.013. Epub 2023 Dec 14.
• Growth pattern of de novo small clusters of colorectal cancer is regulated by Notch signaling at detachment Lin YK, Coppo R, Onuma K, Endo H, Kondo J, Iwabuchi S, Hashimoto S, Itatani Y, Obama K, Inoue M. Cancer Sci.2024 Nov;115(11):3648-3659. doi: 10.1111/cas.16299. Epub 2024 Sep 19.
• 糖鎖バイオマーカーの新展開とレクチン治療左近 太佑，近藤 純平，三善 英知 生化学Journal of Japanese Biochemical Society 2024 96(2): 207-214

2023年

• Terminal fucosylation of haptoglobin in cancer-derived exosomes during cholangiocarcinoma progression. Choi H, Ju S, Kang K, Seo MH, Kim JM, Miyoshi E, Yeo MK, Park SY. Front Oncol. 2023 Apr 24;13:1183442. doi: 10.3389/fonc.2023.1183442. eCollection
• Rapid Chemical Synthesis of Serine Protease Inhibitor Kazal-type 13 (SPINK13) Glycoform by a Combined Method with Glycan Insertion Strategy and Fast-Flow Fmoc SPPSNomura K, Okamoto R, Maki Y, Hayashibara A, Takao T, Fukuoka T, Miyoshi E, Pentelute BL, Kajihara Y. Chemistry. 2023 Jul 26;29(42):e202300646. doi: 10.1002/chem.202300646.
• Metabolic clogging of mannose triggers dNTP loss and genomic instability in human cancer cells Harada Y, Mizote Y, Suzuki T, Hirayama A, Ikeda S, Nishida M, Hiratsuka T, Ueda A, Imagawa Y, Maeda K, Ohkawa Y, Murai J, Freeze HH, Miyoshi E, Higashiyama S, Udono H, Dohmae N, Tahara H, Taniguchi N. Elife.2023 Jul 18;12:e83870. doi: 10.7554/eLife.83870.
• Prognostic significance of serum fucosylated pro-haptoglobin in advanced renal cell carcinoma patients treated with immune checkpoint inhibitors Kato T, Morishita K, Tomiyama E, Hayashibara A, Ishizuya Y, Yamamoto Y, Hatano K, Kawashima A, Fukuhara S, Nonomura N, Miyoshi E, Fujita K. Sci Rep. 2023 Oct 11;13(1):17239. doi: 10.1038/s41598-023-42739-1.
• Development of new colloid osmotic pressure measurement method using ultrafiltration membrane during cardiopulmonary bypass Matsumoto T, Yoshida K, Shinohara T, Miyoshi E, Ueno T. J Extra Corpor Technol.2023 Dec;55(4):167-174. doi: 10.1051/ject/2023028.
• Prohaptoglobin is a possible prognostic biomarker for colorectal cancer Morishita K, Kondo J, Sakon D, Hayashibara A, Tamura I, Shimizu K, Takamatsu S, Murata K, Kamada Y, Miyoshi E. Biochem Biophys Res Commun. 2023 Jun 9;672:72-80. doi: 10.1016/j.bbrc.2023.06.017.
• The specific core fucose-binding lectin Pholiota squarrosa lectin (PhoSL) inhibits hepatitis B virus infection in vitro. Ouchida T, Maeda H, Akamatsu Y, Maeda M, Takamatsu S, Kondo J, Misaki R, Kamada Y, Ueda M, Ueda K, Miyoshi E.Sci Rep. 2023 Apr 15;13(1):6175. doi: 10.1038/s41598-023-28572-6.
• Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells. Muranaka M, Takamatsu S, Ouchida T, Kanazawa Y, Kondo J, Nakagawa T, Egashira Y, Fukagawa K, Gu J, Okamoto T, Kamada Y, Miyoshi E. Int J Mol Sci. 2023 Apr 11;24(8):7037. doi: 10.3390/ijms24087037.
• Twin research shows glycan changes are more susceptible to environmental factors than their carrier glycoproteins. Asuka T, Kamada Y, Morishita K, Fukuoka T, Takamatsu S, Kondo J, Watanabe M, Sakai N, Hayakawa K, Miyoshi E.; Osaka Twin Research Group. Glycoconj J. 2023 Apr;40(2):191-198. doi: 10.1007/s10719-023-10099-7. Epub 2023 Feb 14.
• Establishment of a novel 70K Mac-2 binding protein antibody through screening of fucosylation-related antibodies. Masuda M, Asuka T, Terao N, Nishino S, Ikeda S, Takamatsu S, Kondo J, Miyoshi E.J Biochem. 2023 Feb 10:mvad015. doi: 10.1093/jb/mvad015. Online ahead of print.
• Hepatocyte differentiation from mouse liver ductal organoids by transducing 4 liver-specific transcription factors. Tomofuji K, *Kondo J, Onuma K, Coppo R, Horie H, Oyama K, Miyoshi E, Fukumitsu K, Ishii T, Hatano E, Inoue M. Hepatol Commun. 2023 Apr 14;7(5):e0134. doi: 10.1097/HC9.0000000000000134. eCollection 2023 May 1. *corresponding author
• EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblasts. Wu J, Moriwaki K, Asuka T, Nakai R, Kanda S, Taniguchi M, Sugiyama T, Yoshimura SI, Kunii M, Nagasawa T, Hosen N, Miyoshi E, Harada A. Blood Adv. 2023 Apr 12:bloodadvances.2022008930. doi: 10.1182/bloodadvances.2022008930. Online ahead of print.
• CKAP4 is a potential exosomal biomarker and therapeutic target for lung cancer. Nagoya A, Sada R, Kimura H, Yamamoto H, Morishita K, Miyoshi E, Morii E, Shintani Y, Kikuchi A. Transl Lung Cancer Res. 2023 Mar 31;12(3):408-426. doi: 10.21037/tlcr-22-571. Epub 2023 Mar 17.
• Diagnostic accuracy of enhanced liver fibrosis test for nonalcoholic steatohepatitis-related fibrosis: Multicenter study. Seko Y, Takahashi H, Toyoda H, Hayashi H, Yamaguchi K, Iwaki M, Yoneda M, Arai T, Shima T, Fujii H, Morishita A, Kawata K, Tomita K, Kawanaka M, Yoshida Y, Ikegami T, Notsumata K, Oeda S, Kamada Y, Sumida Y, Fukushima H, Miyoshi E, Aishima S, Okanoue T, Nakajima A, Itoh Y; Japan Study Group of Nonalcoholic Fatty Liver Disease (JSG-NAFLD). Hepatol Res. 2023 Apr;53(4):312-321. doi: 10.1111/hepr.13871. Epub 2023 Jan 10.
• Core Fucosylation Is Required for the Secretion of and the Enzymatic Activity of SOD3 in Nonsmall-Cell Lung Cancer Cells. Ohkawa Y, Kitano M, Maeda K, Nakano M, Kanto N, Kizuka Y, Seike M, Azuma A, Yamaguchi Y, Ookawara T, Miyoshi E, Taniguchi N. Antioxid Redox Signal. 2023 Mar 6. doi: 10.1089/ars.2022.0010. Online ahead of print.
• Tracking the growth fate of single cells and isolating slow-growing cells in human colorectal cancer organoids. Coppo R, Kondo J, Onuma K, Inoue M. STAR Protoc. 2023 Jun 28;4(3):102395. doi: 10.1016/j.xpro.2023.102395. Online ahead of print.
• Inhibition of the bone morphogenetic protein pathway suppresses tumor growth through downregulation of epidermal growth factor receptor in MEK/ERK-dependent colorectal cancer. Shimizu S, *Kondo J, Onuma K, Coppo R, Ota K, Kamada M, Harada Y, Tanaka Y, Nakazawa MA, Tamada Y, Okuno Y, Kawada K, Obama K, Coffey RJ, Fujiwara Y, Inoue M. Cancer Science 2023 Jun 25. doi: 10.1111/cas.15882. Online ahead of print. *corresponding author
• Ex vivo chemosensitivity assay using primary ovarian cancer organoids for predicting clinical response and screening effective drugs. Ito Y, Kondo J, Masuda M, Matsuzaki S, Onuma K, Kanda M, Watanabe Y, Sakaguchi H, Yoshino K, Ueda Y, Kamiura S, Kimura T, Inoue M. Hum Cell. Hum Cell. 2023 Mar;36(2):752-761. doi: 10.1007/s13577-022-00827-w. Epub 2022 Dec 7.
• Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity. Coppo R, Kondo J, Iida K, Okada M, Onuma K, Tanaka Y, Kamada M, Ohue M, Kawada K, Obama K, Inoue M. iScience 2023 Jan 13;26(2):105962. doi: 10.1016/j.isci.2023.105962. eCollection 2023 Feb 17.
• De-differentiation in cultures of organoids from luminal-type breast cancer is restored by inhibition of NOTCH signaling Uematsu H, Saito C, Kondo J, Onuma K, Coppo R, Mori Y, Muto M, Kikawa Y, Tada M, Sugie T, Inoue M. Hum Cell. 2023 Nov;36(6):2099-2112. doi: 10.1007/s13577-023-00975-7.

2022年

• Transcription factor SP1 regulates haptoglobin fucosylation via induction of GDP-fucose transporter 1 in the hepatoma cell line HepG2. Kondo J, Sakata N*, Morishita K, Hayashibara A, Sakon D, Takamatsu S, Asakura N, Suzuki T, Miyoshi E. Biochem Biophys Rep. 2022 Oct 25;32:101372. doi: 10.1016/j.bbrep.2022.101372. eCollection 2022 Dec. *co-first author
• Lewis glycosphingolipids as critical determinants of TRAIL sensitivity in cancer cells Fukuoka T, Moriwaki K, Takamatsu S, Kondo J, Tanaka-Okamoto M, Tomioka A, Semba M, Komazawa-Sakon S, Kamada Y, Kaji H, Miyamoto Y, Inoue M, Bessho K, Miyoshi Y, Ozono K, Nakano H, Miyoshi E. Oncogene. Sep;41(38):4385-4396. doi: 10.1038/s41388-022-02434-3. Epub 2022 Aug 15.
• The scaffold-dependent function of RIPK1 in dendritic cells promotes injury-induced colitis. Moriwaki K, Park C, Koyama K, Balaji S, Kita K, Yagi R, Komazawa-Sakon S, Semba M, Asuka T, Nakano H, Kamada Y, Miyoshi E, Chan FKM. Mucosal Immunol. 2022 Jan;15(1):84-95. doi: 10.1038/s41385-021-00446-y.
• Simultaneous analysis of serum α2,3-linked sialylation and core-type fucosylation of prostate-specific antigen for the detection of high-grade prostate cancer. Hatano K, Yoneyama T, Hatakeyama S, Tomiyama E, Tsuchiya M, Nishimoto M, Yoshimura K, Miyoshi E, Uemura H, Ohyama C, Nonomura N, Fujita K. Br J Cancer. 2022 Mar;126(5):764-770. doi: 10.1038/s41416-021-01637-x.
• Enterococcus spp. have higher fitness for survival, in a pH-dependent manner, in pancreatic juice among duodenal bacterial flora. Itoyama S, Noda E, Takamatsu S, Kondo J, Kawaguchi R, Shimosaka M, Fukuoka T, Motooka D, Nakamura S, Tanemura M, Mitsufuji S, Iwagami Y, Akita H, Tobe T, Kamada Y, Eguchi H, Miyoshi E. JGH Open. 2022 Jan 5;6(1):85-90. doi: 10.1002/jgh3.12703.
• TNF receptor-related factor 3 inactivation promotes the development of intrahepatic cholangiocarcinoma through NF-κB-inducing kinase-mediated hepatocyte transdifferentiation Shiode Y, Kodama T, Shigeno S, Murai K, Tanaka S, Newberg JY, Kondo J, Kobayashi S, Yamada R, Hikita H, Sakamori R, Suemizu H, Tatsumi T, Eguchi H, Jenkins NA, Copeland NG, Takehara T.Hepatology. 2022 Jan 7. doi: 10.1002/hep.32317. Online ahead of print.
• ASURAT: Functional annotation-driven unsupervised clustering of single-cell transcriptomes Iida K, Kondo J, Wibisana JN, Inoue M, Okada M. Bioinformatics. 2022 Aug 4:btac541. doi: 10.1093/bioinformatics/btac541. Online ahead of print.
• Polarity switching of ovarian cancer cell clusters via SRC family kinase is involved in the peritoneal dissemination Kawata M, Kondo J, Onuma K, Ito Y, Yokoi T, Hamanishi J, Mandai M, Kimura T, Inoue M.Cancer Sci. 2022 Jul 18. doi: 10.1111/cas.15493.
• Constitutive Activation of the Tumor Suppressor p53 in Hepatocytes Paradoxically Promotes Non-Cell Autonomous Liver Carcinogenesis Makino Y, Hikita H, Fukumoto K, Sung JH, Sakano Y, Murai K, Sakane S, Kodama T, Sakamori R, Kondo J, Kobayashi S, Tatsumi T, Takehara T. Cancer Res. 2022 Aug 16;82(16):2860-2873. doi: 10.1158/0008-5472.CAN-21-4390.
• Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts Tomofuji K, Fukumitsu K, Kondo J, Horie H, Makino K, Wakama S, Ito T, Oshima Y, Ogiso S, Ishii T, Inoue M, Hatano E.Biomaterials. 2022 Jun 1;287:121614. doi: 10.1016/j.biomaterials.2022.121614.
• Ex vivo chemosensitivity assay using primary ovarian cancer organoids for predicting clinical response and screening effective drugs Ito Y, Kondo J, Masuda M, Matsuzaki S, Onuma K, Kanda M, Watanabe Y, Sakaguchi H, Yoshino K, Ueda Y, Kamiura S, Kimura T, Inoue M. Hum Cell. 2023 Mar;36(2):752-761. doi: 10.1007/s13577-022-00827-w. Epub 2022 Dec 7.

2021年

• Challenges in the Application of Glyco-Technology to Hepatitis B Virus Therapy and Diagnosis. Ouchida T, Takamatsu S, Maeda M, Asuka T, Morita C, Kondo J, Ueda K, Miyoshi E. Viruses. 2021 Sep 17;13(9):1860. doi: 10.3390/v13091860.
• Sweet modification and regulation of death receptor signalling pathway. Moriwaki K, Chan FKM, Miyoshi E. J Biochem. 2021 Sep 7;169(6):643-652. doi: 10.1093/jb/mvab034.
• Branched-chain amino acids protect the liver from cirrhotic injury via suppression of activation of lipopolysaccharide-binding protein, toll-like receptor 4, and signal transducer and activator of transcription 3, as well as Enterococcus faecalis translocation. Eguchi A, Iwasa M, Tamai Y, Tempaku M, Takamatsu S, Miyoshi E, Hasegawa H, Kobayashi Y, Takei Y. Nutrition. 2021 Jun;86:111194. doi: 10.1016/j.nut.2021.111194.
• Rifaximin ameliorates intestinal inflammation in cirrhotic patients with hepatic encephalopathy. Tamai Y, Iwasa M, Eguchi A, Shigefuku R, Kamada Y, Miyoshi E, Takei Y. JGH Open. 2021 Jun 23;5(7):827-830. doi: 10.1002/jgh3.12596.
• Identification of fucosylated haptoglobin-producing cells in pancreatic cancer tissue and its molecular mechanism. Ito N, Yamada M, Morishita K, Nojima S, Motooka K, Sakata N, Asuka T, Otsu R, Takamatsu S, Kamada Y, Mori S, Akita H, Eguchi H, Morii E, Miyoshi E. Glycoconj J. 2021 Feb;38(1):45-54. doi: 10.1007/s10719-020-09970-8.
• Detection of fucosylated haptoglobin using the 10-7G antibody as a biomarker for evaluating endoscopic remission in ulcerative colitis. Motooka K, Morishita K, Ito N, Shinzaki S, Tashiro T, Nojima S, Shimizu K, Date M, Sakata N, Yamada M, Takamatsu S, Kamada Y, Iijima H, Mizushima T, Morii E, Takehara T, Miyoshi E. World J Gastroenterol. 2021 Jan 14;27(2):162-175. doi: 10.3748/wjg.v27.i2.162.
• Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation. Kitano M, Kizuka Y, Sobajima T, Nakano M, Nakajima K, Misaki R, Itoyama S, Harada Y, Harada A, Miyoshi E, Taniguchi N. J Biol Chem. 2021 Jan-Jun;296:100354. doi: 10.1016/j.jbc.2021.100354.
• Serum core-type fucosylated prostate-specific antigen index for the detection of high-risk prostate cancer. Fujita K, Hatano K, Tomiyama E, Hayashi Y, Matsushita M, Tsuchiya M, Yoshikawa T, Date M, Miyoshi E, Nonomura N. Int J Cancer. 2021 Jun 15;148(12):3111-3118. doi: 10.1002/ijc.33517.